Technical Data
Media
MEM Eagle w/Earle’s w/o L-Glutamine, Cysteine, Cystine, Methionine, CaCl2, MgSO4, KH2PO4 (Powder)
M3875-05
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/Liter
Inorganic Salts:
Potassium Chloride400
Sodium Chloride6800
Sodium Phosphate Monobasic•H2O 140
Amino Acids:
L-Arginine•HCl126.4
L-Histidine•HCl•H2O41.9
L-Isoleucine52.5
L-Leucine52.5
L-Lysine•HCl73.06
L-Phenylalanine33.02
L-Threonine47.64
L-Tryptophan10.2
L-Tyrosine36.22
L-Valine46.9
Vitamins:
Choline Chloride1
Folic Acid1
myo-Inositol2
Niacinamide1
D-Pantothenic Acid•Ca1
Pyridoxal•HCl1
Riboflavin0.1
Thiamine•HCl1
Other:
D-Glucose1000
Phenol Red Sodium Salt17
Total:8.9g/Liter
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers.  Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.


Directions per Liter: Dissolve 8.9g in 800-900ml of ddH2O stirring gently until completely solubilized. Heat gentyly with stirring. Add 2.0g/L sodium bicarbonate. Adjust pH of the medium to the desired level with 1N HCL or 1N NaOH. Add additional water to bring the solution to 1L. Sterile filter using a 0.22um membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time

Appearance: Light tan to orange, homogenous, free flowing powder

Solubility: Orange to red, clear, complete

pH: As Reported

Endotoxin: 1EU/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H. et al., J. Biol. Chem. 214:845-847 (1956). 2. Eagle, H. Tissue Culture Association Manual 3:517-520 (1976) 3. Eagle, H. Science 130:432-437 (1959). 4. Eagle, H. Science 122:501 (1955)