Technical Data
Media
MEM Glasgow w/L-Glutamine (Powder)
M3880
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride•H2O264.920
Ferric Nitrate•9H2O0.100
Potassium Chloride400.000
Magnesium Sulfate97.670
Sodium Chloride6400.000
Sodium Phosphate Monobasic107.830
Amino Acids:
L-Arginine•HCl42.000
L-Cystine•2HCI31.290
L-Glutamine292.000
L-Histidine•HCl•H2O21.000
L-Isoleucine52.400
L-Leucine52.400
L-Lysine•HCI73.100
L-Methionine15.000
L-Phenylalanine33.000
L-Threonine47.600
L-Tryptophan8.000
L-Tyrosine•2Na•H2O52.190
L-Valine46.800
Vitamins:
D-Pantothenic Acid•Ca2.000
Choline Chloride2.000
Folic Acid2.000
myo-Inositol3.600
Niacinamide2.000
Pyridoxal•HCI2.000
Riboflavin0.200
Thiamine•HCI2.000
Other:
D-Glucose4500.000
Phenol Red, Sodium0.016
Total:12.553g/L
Glasgow Minimum Essential Medium was originally developed by lan MacPherson and Michael Stoker as a modification of Eagle's medium to study the genetic factors affecting cell competence. The polyoma virus was used to transform four fibroblast clones from a culture of baby hamster kidney cells. The medium was developed by modifying Eagle's BME by adding 10% tryptose phosphate and twice the normal concentration of amino acids and vitamins.


Directions per Liter: Dissolve 12.553g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.75g sodium bicarbonate and stir until dissolved. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to tan, homogenous, free flowing powder

Solubility: Colorless to light yellow, clear, complete

pH: As reported

Endotoxin: 10EU/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. MacPherson, I., Stoker, M.: Polyoma Transformation of Hamster Clones-an Investigation of Genetic Factors Affecting Cell Competence. Virology 16: 147-151 (1961).