Technical Data
Media
MEM Glasgow w/L-Glutamine, Tryptose Phosphate Broth (Powder)
M3880-02
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride•2H2O264.92
Ferric Nitrate•9H2O0.10
Magnesium Sulfate97.67
Potassium Chloride400.00
Sodium Chloride6400.00
Sodium Phosphate, anhy107.83
Amino Acids:
L-Arginine•HCl42.00
L-Cystine•2HCI31.29
L-Glutamine292.00
L-Histidine•HCl•H2O21.00
L-Isoleucine52.40
L-Leucine52.40
L-Lysine•HCI73.10
L-Methionine15.00
L-Phenylalanine33.00
L-Threonine47.60
L-Tryptophan8.00
L-Tyrosine•2Na•H2O52.19
L-Valine46.80
Vitamins:
Choline Chloride2.00
Folic Acid2.00
myo-Inositol3.60
Niacinamide2.00
D-Pantothenic Acid•Ca2.00
Pyridoxal•HCI2.00
Riboflavin0.20
Thiamine•HCI2.00
Other:
D-Glucose4500.00
Phenol Red, Sodium16.0
Tryptose Phosphate Broth3000.00
Total: 15.6g/L
Glasgow Minimum Essential Medium was originally developed by lan MacPherson and Michael Stoker as a modification of Eagle's medium to study the genetic factors affecting cell competence. The polyoma virus was used to transform four fibroblast clones from a culture of baby hamster kidney cells. The medium was developed by modifying Eagle's BME by adding 10% tryptose phosphate and twice the normal concentration of amino acids and vitamins.


Directions per Liter: Dissolve 15.6g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.75g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Off-white to tan, homogenous, free flowing powder

Solubility: Red to orange, clear, complete

pH: As reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. MacPherson, I., Stoker, M.: Polyoma Transformation of Hamster Clones-an Investigation of Genetic Factors Affecting Cell Competence. Virology 16: 147-151 (1961).