Technical Data
Media
MEM Glasgow Modified w/2X-L-Glutamine (Powder)
M3880-05
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride•H2O264.9
Ferric Nitrate•9H2O0.1
Potassium Chloride400
Magnesium Sulfate97.7
Sodium Chloride6400
Sodium Phosphate Monobasic•H2O123.8
Amino Acids:
L-Arginine•HCl42
L-Cystine•2Na28.42
L-Glutamine584.6
L-Histidine•HCl•H2O21
L-Isoleucine52.46
L-Leucine52.46
L-Lysine•HCI73.06
L-Methionine14.92
L-Phenylalanine33.02
L-Threonine47.64
L-Tryptophan8.16
L-Tyrosine36.22
L-Valine
Vitamins:
D-Pantothenic Acid•Ca2
Choline Chloride2
FolicAcid2
myo-Inositol4
Niacinamide2
Pyridoxal•HCI2
Riboflavin0.2
Thiamine•HCI2
Other:
D-Glucose4500
Phenol Red, Sodium17
Total:12.9g/L
Glasgow Minimum Essential Medium was originally developed by lan MacPherson and Michael Stoker as a modification of Eagle's medium to study the genetic factors affecting cell competence. The polyoma virus was used to transform four fibroblast clones from a culture of baby hamster kidney cells. The medium was developed by modifying Eagle's BME by adding 10% tryptose phosphate and twice the normal concentration of amino acids and vitamins.


Directions per Liter: Dissolve 12.9 grams per liter of distilled/deionized (DDI) water. Heat with stirring until completely solubilized. Adjust pH as necessary. If required, add sodium bicarbonate (2.75g/L) with stirring after solution has cooled. Sterilize with 0.2um membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to tan, homogenous, free flowing powder

Solubility: Clear, yellow, complete

pH: As reported

Endotoxin: ~10EU/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. MacPherson, I., Stoker, M.: Polyoma Transformation of Hamster Clones-an Investigation of Genetic Factors Affecting Cell Competence. Virology 16: 147-151 (1961).