Technical Data
MTA1 (Metastasis Associated Protein 1)
Metastasis associated protein 1, or metastasis tumor antigen 1 (MTA1), is expressed in metastatic cells, specifically in mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of gastric, colorectal, esophageal, lung, and hepatocellular carcinomas. The role it plays in metastasis is unclear. MTA1 is widely expressed in normal tissues like brain, ovaries, adrenal glands and virgin mammary glands.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:100-1:1000
Western Blot: 1:30-1:100. Identifies doublets at ~80kDa and a band of unknown origin at ~150kD.
Immunohistochemistry (FFPE): 1:50-1:100 Heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining.
Optimal dilutions to be determined by the researcher.

Positive Controls:
Human T47D cell lysates and rat testis homogenates (WB), human breast cancer tissues (IHC).

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
1ml-20CBlue IceHumanMouse
Not Determined
Synthetic peptide derived from the C-terminal region of the human MTA1 protein
Supplied as a liquid, 0.09% sodium azide.
Recognizes the C-terminal region of the human MTA1 protein. Species Crossreactivity: rat. Species Sequence Homology: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Toh Y, et al. J Biol Chem 269: 22958-22963, 1994. 2. Toh Y, et al. Gene 159: 97-104, 1995. 3. Hamatsu, T, et al. Oncol Rep 10(3): 599-604, 2003.