Technical Data
M3884-78A
mGluR4 (Metabotropic Glutamate Receptor)
Description:
The metabotropic glutamate receptors (mGluRs) can be placed into three groups based on sequence homology, coupling to second messengers and pharmacological profiles. Group III includes mGluR4 and its two splice variants mGluR4a and mGluR4b. mGluR4a is the principal mGluR4 receptor found in the cerebellum, hippocampus and other regions of the brain. mGluR4 is also believed to be a taste receptor for the transduction of mono-sodium glutamate taste.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 0.1-1.0ug/ml
Western Blot: 1-2ug/ml
Immunohistochemistry (Paraf n): 5-10ug/ml; verified for rat only.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRatRabbit
Concentration:
~0.2mg/ml
Immunogen:
Synthetic peptide derived from the C-terminal 200aa of the rat mGluR4 protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the mGluR4a splice variant (Mr=93kD-110kD). Reactivity with other related proteins has not been detected. Species Crossreactivity: human, mouse and rat mGluR4. Reactivity was con rmed on Western Blots using rat brain cell lysate.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bradley, S.R., et al., J. Neurosci. 16: 2044-2056 (1996). 2. Nakanishi, S., Science 258: 597-603 (1992). 3. Tanabe, Y., et al., Neuron 8: 169-179 (1992). 4. Chaudhari, N. and Roper, S.D., Annals NY Acad. Sci. 855: 398-406 (1998).