Technical Data
M3885-48
MHC Class 1
Description:
Like their mammalian counterparts, avian MHC Class I molecules (also known as B-F antigens) consist of a highly polymorphic alpha-chain (Mr ~44kD) non-covalently bound to the invariant beta2-microglobulin subunit (Mr ~12kD). MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.1-3

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation Western Blot, Immunohistochemistry and Immunoaffinity chromatography. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k5K89Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceChickenMouse
Concentration:
~0.5mg/ml
Immunogen:
Chicken MHC Class 1.
Purity:
Purified
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Specificity:
Recognizes a monomorphic epitope on avian MHC Class I molecules (Mr ~68kD). Also reacts with turkey MHC Class I.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Salomonsen, J., K. Skjodt, M. Krone and M. Simonsen. 1987. Immunogenetics 25:373-382. 2. L.B. Moller, J. Kaufman, S. Verland, J. Salomonsen, D. Avila, J.D. Lambris and K. Skjodt. 1991. Immunogenetics 34:110-120. 3. Dunon, D., J. Salomonsen, K. Skjodt, J. Kaufman, and B.A. Imhof. 1990. Dev. Immunol. 1:127-135.