Technical Data
M3885-48B
MHC Class 1 (Biotin)
Description:
Like their mammalian counterparts, avian MHC Class I molecules (also known as B-F antigens) consist of a highly polymorphic alpha-chain (Mr ~44kD) non-covalently bound to the invariant beta2-microglobulin subunit (Mr ~12kD). MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.1-3

Applications:
Flow cytometry 1-3
Immunoprecipitation 1-3
Western blotting
Immunohistochemistry
Immunoaffinity chromatography

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k5K89Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceChickenMouse
Concentration:
~0.5mg/ml
Immunogen:
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with Biotin.
Specificity:
Recognizes a monomorphic epitope on avian MHC Class I molecules (Mr ~68kD). Also reacts with turkey MHC Class I.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Salomonsen, J., K. Skjodt, M. Krone and M. Simonsen. 1987. Immunogenetics 25:373-382.
2. L.B. Moller, J. Kaufman, S. Verland, J. Salomonsen, D. Avila, J.D. Lambris and K. Skjodt. 1991. Immunogenetics 34:110-120.
3. Dunon, D., J. Salomonsen, K. Skjodt, J. Kaufman, and B.A. Imhof. 1990. Dev. Immunol. 1:127-135.