Technical Data
M3885-48D1
MHC Class 1 (MHC Class I)
Description:
Human MHC Class I (HLA-A, B & C) molecules are expressed on nearly all nucleated cells. MHC Class I molecules consist of heterodimers of a highly polymorphic transmembrane chain and an invariant chain. MHC Class I molecules bind to endogenously synthesized peptides of about 8-10aa which are presented to CD8+ T cells. Recent evidence now indicates exogenously derived peptides are also presented by MHC Class I molecules.

Applications:
Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a7H121Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human HSB-2 T cell line
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
Specificity:
Recognizes human MHC Class I. Recognizes a monomorphic Class I epitope on the chain.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. GS Eisenbarth, et al, (1980) J Immunol 124: 1237-1244, 2. PJ Bjorkman & P Parham, (1990) Annu Rev Biochem 59: 253-288, 3. LD Barber & P Parham, (1994) J Exp Med 180: 1191-1194, 4. KL Rock, (1996) Immunol Today 17: 131-137, 5. J Immunol (1985) 135(3): 1752-1759, 6. A Kawana-Tachikawa, A Iwamoto, et al (2002) J Virol 76(23):11982-11988, 7. JE Shaw, RW Lang, et al (2001) J Virol Meth 94: 137-146, 8. RM McCallum, BF Haynes, et al. (1993) Opthalmol Vis Sci 34: 1793-1803