Technical Data
M3885-48E
MHC Class 1 (Biotin)
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Technical Data |
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M3885-48E |
MHC Class 1 (Biotin) |
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Description: Human MHC Class I (HLA-A, B & C) molecules are expressed on nearly all nucleated cells. MHC Class I molecules consist of heterodimers of a highly polymorphic transmembrane a chain and an invariant b chain. MHC Class I molecules bind to endogenously synthesized peptides of about 8-10 amino acids which are presented to CD8+ T cells. Recent evidence now indicates exogenously derived peptides are also presented by MHC Class I molecules. Applications: Suitable for use in Flow Cytometry, ELISA and Immunohistochemistry. Functional Application: Antibody (whole molecule) is cytotoxic in the presence of complement (1). Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Flow Cytometry Analysis: 5x10e5 Ficoll prepared peripheral blood mononuclear cells (PBMC) were washed and incubated 45 minutes on ice with 80ul of M3885-48E at 5ug/ml. Cells were washed twice and incubated with secondary reagent Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.28 log10 fluorescent units when compared to a mouse IgG2a/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5 mg/ml anti-MHC Class I antibody M3885-48D (unconjugated clone 7H121). Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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