Technical Data
M3885-48H
MHC Class 1
Description:
The antigenic agglomerate major histocompatibility complex (MHC) class I molecules present antigenic peptides to CD8-positive T cells. It is now recognized that the products of the MHC genes are antigen-presenting molecules (APM) designed for the presentation of antigen fragments (peptides) to the T-cell receptor, thus participating in the immune response (1). Recently shown is a mechanism of antigen acquisition for cross-presentation that couples the antigen presentation system of two adjacent cells and is lost in most tumors: gap-junction-mediated intercellular peptide coupling for presentation by bystander MHC class I molecules and transfer to professional antigen presenting cells for cross-priming (2). The mammalian vomeronasal organ detects social information about gender, status, and individuality. It has been shown that small peptides that serve as ligands for major histocompatibility complex (MHC) class I molecules function also as sensory stimuli for a subset of vomeronasal sensory neurons located in the basal Gao- and V2R receptor-expressing zone of the vomeronasal epithelium (3).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10
Western Blot (Human, mouse, rat): 1:10,000
Immunoprecipitation: 1:30
Immunohistochemistry (Human, mouse): 1:250-1:500
Immunocytochemistry: 1:250-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG8L759Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to human MHC class 1.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.2, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human MHC Class 1 at ~41kD. Species Crossreactivity: does not recognize mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hedrick SM, et al. Cell 70(2):177-80, 1992.
2. Neijssen J, et al. Nature 434(7029):83-8, 2005.
3. Leinders-Sufall T, et al. Science 306(5698):1033-7, 2004.