Description:
Applications:
• Flow cytometry1,2
• Immunohistochemistry (acetone-fixed, frozen tissue sections only)
• Complement-dependent cytoxicity assay1,2
Recommended Dilution:
Flow Cytometry: 0.2ug/10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2a,k | 5K48 | Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ug | | -20°C | Blue Ice | Mouse | Mouse |
|
| Concentration: |
| ~0.1mg/ml |
| Immunogen: |
| C3H.SW splenocytes |
| Purity: |
| Purified |
| Form |
| Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Conjugated to PE. |
| Specificity: |
| Recognizes the alpha3 domain of H-2Db class I MHC antigen. Crossreacts with the alpha3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Ozato, K., T.H. Hansen, and D.H. Sachs. 1980. Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex. J. Immunol. 125:2473-2477.
2. Ozato, K., and D.H. Sachs. 1981. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126:317-321.
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