Technical Data
M3885-60K
MHC Class 1, H-2Dk (MHC Class I)
Description:
This antibody reacts with the H-2Dk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dk haplotype. H-2Dk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. This antibody weakly cross-reacts with H-2Kd and with cells from mice of the H-2f haplotype. Reactivity with other haplotypes (e.g., a,b,p,q,r,s) has not been reported.

Applications:
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k15-5-5Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceMouseMouse
Concentration:
~0.5mg/ml
Immunogen:
C3H mouse splenocytes
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes H-2Dk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dk haplotype. Weakly cross-reacts with H-2Kd and with cells from mice of the H-2f haplotype. Does not cross react with other haplotypes (e.g., a, b, p, q, r, s).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.