Technical Data
M3885-72A1
MHC Class 1, H-2Kb (MHC Class I H-2Kb, H-2 Class I Histocompatibility Antigen K-B alpha Chain, H-2K(B), H2-K1, H-2K, H2-K, H-2K(d), MGC7052, MGC184092) (FITC)
Description:
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. There are 3 major MHC class I proteins encoded by the H-2 which are H-2K, H-2L and H-2D. The H- 2K gene is part of the murine H-2 complex on chromosome 17 and there are a large number of variant alleles of this gene.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:10-1:20; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2aAF6-88.5Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseMouse
Concentration:
~0.5mg/ml
Immunogen:
C57BL mouse splenocytes
Purity:
Purified by affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes the H-2Kb determinant present on all nucleated cells of mice expressing this MHC class I haplotype. Does not cross react with other haplotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.