Technical Data
MHC Class 1, H-2Kb (MHC Class I H-2Kb, H-2 Class I Histocompatibility Antigen K-B alpha Chain, H-2K(B), H2-K1, H-2K, H2-K, H-2K(d), MGC7052, MGC184092) (FITC)
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. There are 3 major MHC class I proteins encoded by the H-2 which are H-2K, H-2L and H-2D. The H- 2K gene is part of the murine H-2 complex on chromosome 17 and there are a large number of variant alleles of this gene.

Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:10-1:20; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
MabIgG2aAF6-88.5Affinity Purified
100ug-20CBlue IceMouseMouse
C57BL mouse splenocytes
Purified by affinity chromatography
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Recognizes the H-2Kb determinant present on all nucleated cells of mice expressing this MHC class I haplotype. Does not cross react with other haplotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.