Technical Data
M3885-73V
MHC Class 1, H-2Dd, H-2Kb (MHC Class I, H-2Dd, H-2Kb)
Description:
This antibody reacts with the H-2Kb/H-2Dd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kb/H-2Dd haplotype. H-2Kb/H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. This antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.25ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k34-1-2SAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouseMouse
Concentration:
~0.5mg/ml
Immunogen:
BDF Splenocytes
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes mouse H-2Kd/H-2Dd. Crossreactivity: H-2Kb, s, r, q, p.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.