Technical Data
M3885-80B
MHC Class 1, H-2Kb (MHC Class I H-2Kb, H-2 Class I Histocompatibility Antigen K-B alpha Chain, H-2K(B), H2-K1, H-2K, H2-K, H-2K(d), MGC7052, MGC184092)
Description:
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. There are 3 major MHC class I proteins encoded by the H-2 which are H-2K, H-2L and H-2D. The H- 2K gene is part of the murine H-2 complex on chromosome 17 and there are a large number of variant alleles of this gene.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10-1:20. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: Frozen
Optimal working dilutions to be determined by researcher.

Storage and Stability:

May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

TypeIsotypeCloneGrade
MabIgG2aAF6-88.5Affinity Purified
SizeStorageShippingSourceHost
250ug-20CBlue IceMouseMouse
Concentration:
~0.5mg/ml
Immunogen:
Mouse MHC Class 1, H2, Kb
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes the mouse MHC Class I H-2Kb antigen. Does not cross react with other haplotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.