		Technical Data
M3885-98	MHC Class 1, H-2Kq (MHC Class I, H-2Kq)

Description:
This antibody reacts with the H-2Kq MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kq haplotype. H-2Kq is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. This antibody has not been reported to cross-react with other haplotypes.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.25ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a,k		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Mouse	Mouse

Concentration:

~0.5mg/ml

Immunogen:

B10.Q mouse splenocytes

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Specificity:

Recognizes mouse q haplotype. Does not cross-react with other haplotypes (e.g. a, b, d, f, k, n, p, r, s, u, v).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369. 4. Hasendrug K, et al. 1987. Immunogenetics 25:136.