Technical Data
M3886-10
MHC Class 1 HLA ABC
Description:
Human MHC class I antigens are expressed constitutively on all nucleated cells such as lymphocytes, thymocytes, granulocytes and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance and tissue allotransplantation. This clone also reacts strongly with primate cells.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Positive Control:
Human lymphocytes, Tonsil and lymph nodes

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k0.N.414Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Human peripheral blood leukocytes. Cellular Localization: Cell membrane.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M3886-10X.
Specificity:
Recognizes a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Species Crossreactivity: primates
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application references: 1. Nozaki, K. and Pestronk, A. (2009) J Neurol Neurosurg Psychiatry 80:904-908. 2. Miller, T. et al., (2002) J Neurol Neurosurg Psychiatry 73:420-428. 3. Temiz, P. et al., (2009) J Neurol Sci. 278:25-29. 1. Young, N.T., et al., Hum. Immunol. 52: 1-11 (1997). 2. Krensky, A.M., et al., Transplant Proc. 28: 3026-3028 (1996). 3. Hansen, J.A., et al., Hematol. Oncol. Clin. North Am. 4: 507-515 (1990).