Human MHC class I antigens are expressed constitutively on all nucleated cells such as lymphocytes, thymocytes, granulocytes and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance and tissue allotransplantation. This clone also reacts strongly with primate cells.
Suitable for use in Flow Cytometry. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Human lymphocytes, Tonsil and lymph nodes
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Human peripheral blood leukocytes. Cellular Localization: Cell membrane.|
|Purified by Protein A affinity chromatography.|
|Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M3886-10X.|
|Recognizes a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Species Crossreactivity: primates|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
US Biological application references: 1. Nozaki, K. and Pestronk, A. (2009) J Neurol Neurosurg Psychiatry 80:904-908. 2. Miller, T. et al., (2002) J Neurol Neurosurg Psychiatry 73:420-428. 3. Temiz, P. et al., (2009) J Neurol Sci. 278:25-29. 1. Young, N.T., et al., Hum. Immunol. 52: 1-11 (1997). 2. Krensky, A.M., et al., Transplant Proc. 28: 3026-3028 (1996). 3. Hansen, J.A., et al., Hematol. Oncol. Clin. North Am. 4: 507-515 (1990).|