Technical Data
M3886-12
MHC Class 1 HLA ABC
Description:
Human MHC class I antigens (HLA-A, B and C) are expressed constitutively on all nucleated cells such as lymphocytes, thymocytes, granulocytes, and bone marrow cells. They are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen only. Acetone fixation recommended.
Optimal dilutions to be determined by the researcher.

Positive Control:
Human lymphocytes. Tonsil and lymph node.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a3F273Affinity Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Cell membranes of human tonsil lymphocytes.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M3886-12X.
Specificity:
Recognizes a monomorphic determinant of human major histocompatibility (MHC) class I antigens.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Appleyard, S.T., et al., Lancet 1: 361–363 (1985). 2. Daar, A.S., et al., Transplantation 38: 287–292 (1984). 3. Muller, C., et al., Eur. J. Immunol. 13: 414–418 (1938).