Technical Data
M3886-55A
MHC Class 1, H2 Dd (H-2 Class I Histocompatibility Antigen, D-D alpha Chain, H-2D(D), H2-D1)
Description:
Recognizes a3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype. H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The M3886-55A cross-reacts with cells from the C3H.LG/Ckc strain. Reactivity with other haplotypes (e.g., b,f,k,p,q,r,s) has not been reported.

Applications:
Suitable for use in Immunoprecipitation, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.5ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k34-2-12Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouseMouse
Concentration:
~0.1mg/ml
Immunogen:
C57BL/6 x DBA/2)F1 mouse splenocytes
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes mouse d haplotype.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ozato, K., et al., 1982. Transplantation 34:113. 2. McCluskey, J., et al., 1985. Cell 40:247. 3. McCluskey, J., et al., 1986. J. Immunol. 136:1472. 4. Kane, K.P. 1994. J. Exp. Med. 179:1011. 5. Daniels, B.F., et al., 1994. J. Exp. Med. 180:687. 6. Nikolic , B., et al., 1999. J. Immunol. 162:3402. 7. Ma, X-T., et al., 2006. Cancer Research 66:1169.