		Technical Data
M3886-55B	MHC Class 1, H2 Dd (H-2 Class I Histocompatibility Antigen, D-D alpha Chain, H-2D(D), H2-D1) (Biotin)

Description:
Recognizes a3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype. H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. M3886-55B cross-reacts with cells from the C3H.LG/Ckc strain. Reactivity with other haplotypes (e.g., b,f,k,p,q,r,s) has not been reported.

Applications:
Suitable for use in Immunoprecipitation, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.5ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a,k	34-2-12	Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Mouse	Mouse

Concentration:

~0.1mg/ml

Immunogen:

C57BL/6 x DBA/2)F1 mouse splenocytes

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.

Specificity:

Recognizes mouse d haplotype.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Ozato, K., et al., 1982. Transplantation 34:113. 2. McCluskey, J., et al., 1985. Cell 40:247. 3. McCluskey, J., et al., 1986. J. Immunol. 136:1472. 4. Kane, K.P. 1994. J. Exp. Med. 179:1011. 5. Daniels, B.F., et al., 1994. J. Exp. Med. 180:687. 6. Nikolic , B., et al., 1999. J. Immunol. 162:3402. 7. Ma, X-T., et al., 2006. Cancer Research 66:1169.