Technical Data
M3886-55C
MHC Class 1, H-2Dd (MHC Class I, H-2Dd)
Description:
This antibody recognizes a3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype. H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. This antibody cross-reacts with cells from the C3H.LG/Ckc strain. Reactivity with other haplotypes (e.g., b,f,k,p,q,r,s) has not been reported. This clone recognizes the a3 domain of Dd.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.25ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k34-2-12Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceMouseMouse
Concentration:
~0.5mg/ml
Immunogen:
(C57BL/6 x DBA/2)F1 mouse splenocytes
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes mouse d haplotype. Crossreactivy: C3H.LG/Ckc. Does not cross react with other haplotypes (e.g., b, f, k, p, q, r, s).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.