		Technical Data
M3886-75A	MHC Class 1 Chain-related Gene A (MHC Class I Polypeptide-related Sequence A, MICA, MIC-A, FLJ60820, MGC111087, PERB11.1)

Description:
MICA is the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow cytometry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Flow cytometry: 1:10-1:50
Immunofluorescence: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide corresponding to the center region, between aa75-105, of human MICA conjugated to KLH.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human MICA.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Bahram,S., et.al., Proc. Natl. Acad. Sci. USA 91(14), 6259-6263 (1994). 2.Klein,J.et.al., Proc. Natl. Acad. Sci. USA 91(14), 6251-6252 (1994). 3.Parham,P., et.al., J. Immunol. 142(11), 3937-3950 (1989).