Technical Data
M3886-75A
MHC Class 1 Chain-related Gene A (MHC Class I Polypeptide-related Sequence A, MICA, MIC-A, FLJ60820, MGC111087, PERB11.1)
Description:
MICA is the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunohistochemistry: 1:10-1:50
Flow Cytometry: 1:10-1:50
Immunofluorescence: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to the center region, between corresponding to a portion of amino acids within aa 68-97, of human MHC Class 1 Chain-related Gene A conjugated to KLH (Q29983).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human MHC Class 1 Chain-related Gene A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Bahram,S., et.al., Proc. Natl. Acad. Sci. USA 91(14), 6259-6263 (1994). 2.Klein,J.et.al., Proc. Natl. Acad. Sci. USA 91(14), 6251-6252 (1994). 3.Parham,P., et.al., J. Immunol. 142(11), 3937-3950 (1989).