MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells.
MICA is principally expressed on intestinal epithelium, and several epithelial tumours. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection.
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul
Immunohistochemistry: Frozen sections. Membrane permeabilization is required.
Optimal dilutions to be determined by the researcher.
P3 myeloma cells with spleen cells from RBF/DnJ mice.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|MICA transfected C1R cells|
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.|
|Recognizes human nonclassical MHC class I chain A (MICA) and nonclassical MHC class I chain MICB (MICB). Reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
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