Technical Data
M3886-75Y2
MHC Class 1 Chain-related Gene A, B (MHC Class 1 Chain-related Gene A, MHC Class I Polypeptide-related Sequence A, MICA, MIC-A, MHC Class 1 Chain-related Gene B, MHC Class I Polypeptide-related Sequence B, MICB, MIC-B, PERB11.1, PERB11.2) (Biotin)
Description:
MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells.
MICA is principally expressed on intestinal epithelium, and several epithelial tumours. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul
Immunohistochemistry: Frozen sections. Membrane permeabilization is required.
Optimal dilutions to be determined by the researcher.

Hybridoma:
P3 myeloma cells with spleen cells from RBF/DnJ mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a10F158Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
MICA transfected C1R cells
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
Specificity:
Recognizes human nonclassical MHC class I chain A (MICA) and nonclassical MHC class I chain MICB (MICB). Reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Groh, V.et al. (1998) Recognition of stress-induced MHC molecules by intestinal epithelial gamma delta T cells. Science. 279: 1737-1740. 2. Bauer, S. et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 285: 727-729. 3. Groh, V. et al. (1999) Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB. Proc. Natl. Acad. Sci. USA. 96: 6879-6884. 4. Groh, V. et al. (2001) Costimulation of CD8 alpha beta T cels by NKG2D via engagement by MIC induced on virus-infected cells. Nat Immunol. 2: 255-60. 5. Das, H. et al. (2004) Mechanisms of V delta 1 T cell activation by microbial components. J. Immunol. 172: 6578-6586. 6. Jinushi, M. et al. (2003) Critical role of MHC class I related chain A and B expression on IFN alpha stimulated dendritic cells in NK cell activation: Impairment in chronic hepatitis C virus infection. J. Immunol. 170: 1249-1256. 7. Groh, V. et al. (2003) Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis. Proc Natl Acad Sci U S A. 100: 9452-7. 8. Jinushi, M. et al. (2003) Autocrine/paracrine IL-15 that is required for type I IFN-mediated dendritic cell expression of MHC class I-related chain A and B is impaired in hepatitis C virus infection. J Immunol. 171: 5423-9. 9. Park, E.J. et al. (2003) Clonal expansion of double-positive intraepithelial lymphocytes by MHC class I-related chain A expressed in mouse small intestinal epithelium. J Immunol. 171: 4131-9. 10. Wu, J. et al. (2003) Intracellular retention of the MHC class I-related chain B ligand of NKG2D by the human cytomegalovirus UL16 glycoprotein. J Immunol. 170: 4196-200. 11. Xu, X. et al. (2006) Clinicopathological significance of major histocompatibility complex class I-related chain a and B expression in thyroid cancer. J Clin Endocrinol Metab. 91: 2704-12. 12. Shi, J. et al. (2008) Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma. Blood. 111: 1309-17.