		Technical Data
M3886-75Y3	MHC Class 1 Chain-related Gene A, B (MHC Class 1 Chain-related Gene A, MHC Class I Polypeptide-related Sequence A, MICA, MIC-A, MHC Class 1 Chain-related Gene B, MHC Class I Polypeptide-related Sequence B, MICB, MIC-B, PERB11.1, PERB11.2) (FITC)

Description:
MHC Class 1 Chain-related Gene A, B (MICA, MICB) are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumours. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul. Requires membrane permeabilization.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized RBF/DnJ mice were fused with cells of the P3 myeloma cell line.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Mab	IgG2a	10F158	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

MICA transfected C1R cells

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

Specificity:

Recognizes human nonclassical MHC Class 1 Chain A (MICA) and nonclassical MHC class I chain MICB (MICB).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

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