Technical Data
M3887-30
MHC Class 2 Ia (HLA DR)
Description:
HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of an a chain (36 kDa) and a beta chain (27kD). It is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They therefore have a critical role in the initiation of the immune response.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen and bouin or paraffin): 2-4ug/ml for 30 minutes at RT.
Optimal dilution to be determined by researcher.

Positive Control:
Tonsil/lymph node

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b0.N.422Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Activated human peripheral blood mononuclear cells. Cellular Localization: Cell membrane. MW of Antigen: 36kD (alpha chain) and 27kD (beta chain).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity:
Recognizes a human non-polymorphic antigen of the HLA-DR, Ia region. Reacts with B-cells of the germinal centers and mantle zones. It also recognizes monocytes, macrophages and interdigitating histiocytes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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