Technical Data
M3890-03A
b2-Microglobulin (b2M)
Description:
Beta-2-microglobulin (B2M) is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning, and other conditions leading to renal tubular dysfunction. Like immunoglobulins, prealbumin, and the beta protein found in the amyloid of Alzheimer disease, B2M has a predominantly beta-pleated sheet structure that may adopt the fibrillar configuration of amyloid in certain pathologic states. The protein is a single polypeptide chain of molecular weight 11,600 (human 119aa, chromosome 15). B2M associates with heavy chain of class I MHC antigens on the cell surface. A transient complex of MHC-heavy chain and B2M is known to be assembled into the TAP molecule involving interation with a number of chaperones. Binding of the processed peptide releases the class I-B2M complex to the cell surface. Absence of binding leads to degradation in the proteasome. Progressive hepatic iron overload, indistinguishable from that observed in HFE, was found only in mice homozygous for the mutated B2M gene.

Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1ug/ml
Western Blot: 2-4ug/ml
Immunohistochemistry (Paraffin): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a6D585Highly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified human b2-microglobulin.
Purity:
Purified by DEAE chromatography. ( 95% by SDS-PAGE)
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:
Recognizes human b2-microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as soluble human b2-microglobulin. Does not cross-react with mouse, bovine, rabbit, canine or chicken b2-microglobulin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
I. Hilgert, et al.;Folia Biol. (Praha)30,369(1984).