Technical Data
M3891
Microphthalmia (Transcription Factor, MiTF)
Description:
Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kD and 56kD, while the longer Mi form runs as a cluster of bands at 60-70kD in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart (1).

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Gel Supershift: Use M3891X
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (formalin/paraffin): 2-4ug/ml for 30 min at RT.

Optimal dilutions to be determined by the researcher.

Positive Control:
501 Mel cells (1). Melanoma

Cellular Localization:
Nuclear

MW of Antigen:
52-56kD doublet

Epitope:
N-terminal

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k3F275Affinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
N-terminal fragment of human Mi protein. Cellular localization: nuclear.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M3891X.
Specificity:
Recognizes human Microphthalmia with 52-56kD doublet. Does not crossreact with other b-HLH-ZIP factors by DNA mobility shift assay. Reacts with both melanocytic and non-melanocytic isoforms of Mi. It is especially useful for research on mouse Mi protein. Species Crossreactivity: equine, canine, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Weilbaecher, K.N., et al., J. Exp. Med. 187: 775-785 (1998). 2. Hemesath, P., et al., Nature 391: 298-301 (1998).