Technical Data
Media
Middlebrook 7H10 Mycobacteria Agar (Powder)
M3895
Microbiological Grade
Storage RT/4C    Shipping RT
Formulation per Liter g/Liter
Components
Agar15.0
Ammonium Sulfate0.5
Biotin0.0005
Calcium Chloride0.0005
Copper Sulfate0.001
Ferric Ammonium Citrate0.04
L-Glutamic Acid0.5
Magnesium Sulfate0.025
Malachite Green0.00025
Sodium Citrate0.4
Potassium Phosphate Dibasic1.5
Potassium Phosphate Monobasic1.5
Pyridoxine0.001
Zinc Sulfate0.001
Total:19.47g/Liter
Used as a medium for cultivation of Mycobacterium sp. when supplemented with glycerol and OADC. Modification of Middlebrook 7H9 or 7H11 formulations.


Directions: Suspend 19.47g in 900 ml of distilled water. Add 5 ml of glycerol. Boil to dissolve the medium completely. Distribute in180 ml amounts in erlenmeyer flasks and sterilize by autoclaving at 15 lbs. pressure (121C) for 10 minutes. Cool to 45C and aseptically add 100 ml of Middlebrook OADC Growth Supplement. Mix well and pour into screw capped tubes. Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated protected from light, and used within a short period of time.

Expected Cultural Response (2-4 weeks at 35-37C): Mycobacterium smegmatis ATCC 14468

Appearance: Tan to light green, homogeneous, free-flowing powder

Solubility: Pale yellow to medium amber with slight green tint, slightly opalescent with heat.

pH: As Reported

Endotoxin: ~10Eu/mg

Gel: Gelling


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1.APJH, 98:844 (1968) 2. Am. Rev. Resp. Dis., 98:925 (1968)