Technical Data
Media
Middlebrook 7H10 Mycobacteria Agar (Powder)
M3895
Microbiological Grade
Storage RT/4°C    Shipping RT
Formulation per Liter g/Liter
Components
Agar15.0
Ammonium Sulfate0.5
Biotin0.0005
Calcium Chloride0.0005
Copper Sulfate0.001
Ferric Ammonium Citrate0.04
L-Glutamic Acid0.5
Magnesium Sulfate0.025
Malachite Green0.00025
Sodium Citrate0.4
Potassium Phosphate Dibasic1.5
Potassium Phosphate Monobasic1.5
Pyridoxine0.001
Zinc Sulfate0.001
Total:19.47g/Liter
Used as a medium for cultivation of Mycobacterium sp. when supplemented with glycerol and OADC. Modification of Middlebrook 7H9 or 7H11 formulations.


Directions: Suspend 19.47g in 900 ml of distilled water. Add 5 ml of glycerol. Boil to dissolve the medium completely. Distribute in180 ml amounts in erlenmeyer flasks and sterilize by autoclaving at 15 lbs. pressure (121°C) for 10 minutes. Cool to 45°C and aseptically add 100 ml of Middlebrook OADC Growth Supplement. Mix well and pour into screw capped tubes. Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated protected from light, and used within a short period of time.

Expected Cultural Response (2-4 weeks at 35-37°C): Mycobacterium smegmatis ATCC 14468

Appearance: Tan to light green, homogeneous, free-flowing powder

Solubility: Pale yellow to medium amber with slight green tint, slightly opalescent with heat.

pH: As Reported

Endotoxin: ~10Eu/mg

Gel: Gelling


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1.APJH, 98:844 (1968) 2. Am. Rev. Resp. Dis., 98:925 (1968)