Technical Data
Media
Middlebrook 7H9 Mycobacteria Base (Powder)
M3895-05
Microbiological Grade
Storage RT/4°C    Shipping RT
Formulation per Liter g/Liter
Components
Ammonium Sulfate0.5
Biotin0.0005
Calcium Chloride0.0005
Copper Sulfate0.001
Ferric Ammonium Citrate0.04
L-Glutamic Acid0.5
Magnesium Sulfate0.05
Potassium Phosphate Dibasic2.5
Potassium Phosphate Monobasic1.0
Pyridoxine0.001
Sodium Citrate0.1
Zinc Sulfate0.001
Total:4.7g/Liter
Used as a medium for cultivation of Mycobacterium sp. when supplemented with glycerol and OADC. Modification of Middlebrook 7H10 or 7H11 formulations.


Directions per Liter: Dissolve 4.7grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light beige to beige, homogeneous, free flowing powder Solubility

Typical Growth Analysis: Mycobacterium kansasii Group I ATCC 12478 Mycobacterium scrofulaceum Group II ATCC 19981 Mycobacterium intracellulare Group III ATCC 13950 Mycobacterium fortuitum Group IV ATCC 6841 Mycobacterium tuberculosis H37Ra ATCC 25177


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.