Technical Data
Middlebrook 7H9 Mycobacteria Base ADC Enrichment Medium (Powder)
Microbiological Grade
Storage 4C    Shipping RT
ADC Enrichment medium is used with Middlebrook 7H9 Mycobacteria Base(Cat#M3895-05).
Formulation per Literg/Liter
Bovine Albumin (Fraction V)50.00
Sodium Chloride8.50
Total: 78.53
Not Included. See M3895-05
Middlebrook 7H9 Mycobacteria Base
Potassium Phosphate Dibasic2.5
Potassium Phosphate Monobasic1.0
Ammonium Sulfate0.5
Sodium Citrate0.1
Magnesium Sulfate0.05
Calcium Chloride0.0005
Zinc Sulfate0.001
Copper Sulfate0.001
L-Glutamic Acid0.5
Ferric Ammonium Citrate0.04
Final pH (25C): 6.6 0.2
ADC Enrichment Medium is used as a supplement for Middlebrook 7H9 Mycobacteria Base which is used as a medium for cultivation of Mycobacterium sp. when supplemented with glycerol and OADC. Modification of Middlebrook 7H10 or 7H11 formulations.

Note: This product is sold as a powder.

Directions per Liter: To prepare 100ml, dissolve 7.853g in 100ml ddH2O. Filter-sterilize using a 0.22 micron membrane filter. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.Add 100ml of the sterile-filtered solution to 900ml of M3895-05 (Middlebrook 7H9 Mycobacteria Base).

Storage and Stability:Store powdered medium at 4C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared medium should be kept at 4C and used within a short period of time.

Appearance: Off white to yellow, homogeneous, free-flowing powder

Solubility: Colorless to light yellow, clear, complete

pH: As Reported

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. APJH, 98:844 (1968). 2. Am. Rev. Resp. Dis., 98:925 (1968).