Technical Data
Migration Inhibitory Factor, Recombinant, Human (Macrophage MIF, Glycosylation-Inhibiting Factor, GIF, GLIF, MMIF)
Molecular Biology Storage: -20CShipping: Blue Ice
Macrophage migration inhibitory activity originally identified 30 years ago based on the ability to prevent the migration of guinea pig macrophages out of capillary tubes in vitro. A number of cytokines, including interferon-g and IL-4, exhibit macrophage migration inhibitory activity. A cDNA clone was isolated from a lectin-stimulated T-cell hybridoma cDNA library and reported to encode a protein with macrophage migration inhibitory activity. This protein was referred to as human migration inhibitory factor (MIF). MIF cDNA encodes a 115 amino acid residue polypeptide that lacks conventional hydrophobic leader sequence. MIF is expressed in many tissues and in all cell lines examined to date. MIF was also discovered as a growth factor-induced delayed early response gene DER6. Recently, MIF was reported to be a secreted cytokine that exerts a central and wide ranging role in host inflammatory responses. Evidence for a receptor of this has not been reported. E. coli-expressed MIF lacks macrophage migration inhibitory activity and is recommended for use as a calibrator for MIF immunoassays.

Purified recombinant human MIF contains 114 amino acid residues and lacks the N-terminal methionyl group. Recombinant MIF has a predicted molecular mass of approximately 12.5kD.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile PBS,,
> 0.1% BSA or HSA. Aliquot and store at -20C. Reconstituted product is stable for 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Human, recombinant protein expressed in E. coli.
Purity: ~ 97% as determined by N-terminus analysis and SDS-PAGE (silver stain).
Form: Supplied as a lyophilized powder from 100mM Tris, pH 8.0 and 50ug BSA/ug protein. Reconstitute with sterile PBS, > 0.1% BSA or HSA to 10ug/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.