Technical Data
M3895-07L
Migration Inhibitory Factor, Macrophage, Recombinant, Human (Macrophage MIF, Glycosylation-Inhibiting Factor, GIF, GLIF, MMIF)
100ug
Growth Factors, Cytokines Storage: -20CShipping: Dry Ice
The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.

Source:
Recombinant corresponding to aa1-115 of human Macrophage MIF expressed in
E. coli (NP_002406).

Sequence
MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC
SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA

Molecular Weight: 12kD (115 aa)

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
12kD
Source: E .coli.
Purity: ~98% by SDS PAGE.
Concentration: ~1mg/ml
Form: Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 0.5mM DTT, 10% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Weiser WY., et al (1989) Proc Natl Acad Sci. 86: 7522-7526. 2. Bernhagen J., et al (1994) Biochemistry. 33: 14144-14155. 3. Richard B., (1996) FASEB J. 10: 1607-1613.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.