		Technical Data
M3896-03	Midkine, Recombinant, Human (MDK, MK, Neurite Growth Promoting Factor-2, NEGF-2)	100ug

Growth Factors, Cytokines

Storage: -20°CShipping: Blue Ice

Midkine, also known as Neurite Growth-promoting Factor 2, is a basic heparin-binding growth factor of low molecular weight, a member of the NEGF family whose founding member is pleiotrophin. This protein exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Midkine is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. Recombinant human Midkine protein, fused to His-tag at N-terminus, , expressed in E. coli and purified by using conventional chromatography.

Source:
Recombinant human Midkine, aa21-143, His-tagged, expressed in E. coli

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MVAKKKDKVK KGGPGSECAE WAWGPCTPSS KDCGVGFREG TCGAQTQRIR CRVPCNWKKE FGADCKYKFE NWGACDGGTG TKVRQGTLKK ARYNAQCQET IRVTKPCTPK TKAKAKAKKG KGKD

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
15.7kD

Source: E.coli
Purity: ~90% by SDS-PAGE Purified by immunoaffinity chromatography.
Form: Supplied as liquid in 20mM Tris-HCl buffer, pH8.0, 0.1 M NaCl 2mM DTT, and 20% Glycerol

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Mashima T., et al. (2009). Oncogene. 28(1):9-19. 2. Jin Z., et al. (2008). Clin Cancer Res. 14(16):5033-42.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.