Technical Data
Media
Mitis Salivarius Agar (Powder)
M3975
Microbiological Grade
Storage RT/4C    Shipping RT
Components (g/liter):
Agar15.0
Casein Digest Peptone15.0
Peptic Digest of Animal Tissue5.0
Dipotassium Phosphate4.0
Crystal Violet0.0008
Sucrose50.0
Dextrose1.0
Trypan Blue 0.075
Total:90g
Appearance:
Light bluish beige, homogeneous, free flowing powder

Solubility: Deep royal blue, trace to slightly hazy

pH:
7.00.4

Microbiological Tests:
Cultures were incubated at 35C and examined for growth at 18-48 hours

Escherichia coli ATCC 25922: Partial to complete inhibition
Staphylococcus aureus ATCC 25923: Partial to complete inhibition
Streptococcus mitis ATCC 9811: Growth; blue colonies
Streptococcus pyogenes ATCC 19615: Growth; blue colonies
Streptococcus salivarius ATCC 13419: Growth; blue gum drop colonies

Formulation (g/L):
Casein digest of Peptone: 15g
Peptic digest of Animal Tissue: 5g
Sucrose: 50g
Dextrose: 1g
Dipotassium Phosphate: 4g
Trypan Blue: 0.075g
Crystal Violet: 0.0008g
Agar: 15g

Directions per Liter:
Dissolve 90 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi). Cool to 45-50C and aseptically add 1ml of a 1% potassium tellurite solution.

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Directions: Mix 90 grams of the medium in a Liter of purified water until evenly dispersed. Heat with repeated stirring and bring to a boil to dissolve completely. Distribute and autoclave at 121C for 15 minutes. Cool the sterilized medium to 50-60C and aseptically add 1 ml of a 1% potassium tellurite solution.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.