Technical Data
Media
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Technical Data
Media
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Mitis Salivarius Agar (Powder)
M3975
Microbiological Grade
Storage RT/4°C Shipping RT
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Components (g/liter):
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Appearance: Light bluish beige, homogeneous, free flowing powder Solubility: Deep royal blue, trace to slightly hazy pH: 7.0±0.4 Microbiological Tests: Cultures were incubated at 35ºC and examined for growth at 18-48 hours Escherichia coli ATCC 25922: Partial to complete inhibition Staphylococcus aureus ATCC 25923: Partial to complete inhibition Streptococcus mitis ATCC 9811: Growth; blue colonies Streptococcus pyogenes ATCC 19615: Growth; blue colonies Streptococcus salivarius ATCC 13419: Growth; blue “gum drop” colonies Formulation (g/L): Casein digest of Peptone: 15g Peptic digest of Animal Tissue: 5g Sucrose: 50g Dextrose: 1g Dipotassium Phosphate: 4g Trypan Blue: 0.075g Crystal Violet: 0.0008g Agar: 15g Directions per Liter: Dissolve 90 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). Cool to 45-50ºC and aseptically add 1ml of a 1% potassium tellurite solution. Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time. Directions: Mix 90 grams of the medium in a Liter of purified water until evenly dispersed. Heat with repeated stirring and bring to a boil to dissolve completely. Distribute and autoclave at 121°C for 15 minutes. Cool the sterilized medium to 50-60°C and aseptically add 1 ml of a 1% potassium tellurite solution. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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