Technical Data
Murine anti-mitochondria monoclonal antibody produces a "spaghetti"-like staining pattern in the cytoplasm of normal and malignant human cells. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker of the mitochondria in subcellular fractions. This monoclonal antibody is part of a new panel of reagents which recognize subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues and biochemical preparations.

Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry:(formalin/paraffin) 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cells, Liver

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG10.N.424Affinity Purified
500ul-20CBlue IceHumanMouse
Semi-purified mitochondrial preparation. Cellular Localization: Cytoplasmic.
Purified by Protein G affinity chromatography.
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also supplied without BSA and sodium azide. See M3985X.
Recognizes a human 60kD non-glycosylated protein component of mitochondria found in human cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.