Technical Data
M3985
Mitochondria
Description:
Murine anti-mitochondria monoclonal antibody produces a "spaghetti"-like staining pattern in the cytoplasm of normal and malignant human cells. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker of the mitochondria in subcellular fractions. This monoclonal antibody is part of a new panel of reagents which recognize subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues and biochemical preparations.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry:(formalin/paraffin) 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cells, Liver

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.N.424Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Semi-purified mitochondrial preparation. Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also supplied without BSA and sodium azide. See M3985X.
Specificity:
Recognizes a human 60kD non-glycosylated protein component of mitochondria found in human cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.