Technical Data
Presenilin 1 is a protein found in brain cells. Presenilin 1 has a major influence on the risk of contracting alzheimer's disease. Its function is unknown. However, it may be involved in protein production and trafficking, especially during early development. More than 40 different mutations have been found in the presenilin 1 gene, and these mutations associate with early-onset familial alzheimer's disease. Mutations in presenilin 1 tend to elevate levels of amyloid beta in the blood, cerebrospinal fluid, and brains of those affected by mutations

Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:500-1:1000
Immunocytochemistry: 1:100-1:300
Optimal dilutions to be determined by the researcher.

Recommended Positive Controls:
COS7, HeLa, 293 and SK-N-MC cell lines.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not Determined
Presenilin 1 peptide conjugated to KLH
Serum (serum has been preabsorbed against the immunogen peptide)
Supplied as a liquid, 0.01% sodium azide.
Recognizes human Mitochondria at 62kD in Western Blot of subcellular fractions enriched in mitochondrial fractions. Shows a moderate titer by ELISA to the immunogen peptide, but no presenilin 1 bands are observed by Western Blot. Immuno- cytochemistry with M3985-05 revealed a mitochondria-like staining that was still present after exhaustive preabsorption with the peptide. The staining co-localizes with M3985-06. Species Crossreactivity: monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.