Technical Data
M3985-07
Mitochondria
Description:
This monoclonal antibody is part of a new panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb-2 produces a spaghetti-like staining pattern in the cytoplasm of normal and malignant human cells and may be used to stain the mitochondria in paraformaldehyde fixed frozen and formalin-fixed, paraffin-embedded tissues.

Cellular Localization: Immunohistochemically, this antibody produces a spaghetti like staining pattern in the cytoplasm of normal and malignant human cells.

Positive Control: HeLa cells. Liver tissue sections.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry FFPE/Fr. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50%glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18L782Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Semi purified mitochondrial preparation. Epitope: This antibody recognizes a 60kD non glycosylated protein component of mitochondria found in human cells.
Purity:
Purified by Protein G affinity chromatography.
Form
10mM PBS [pH7.4] with 0.2%BSA
Specificity:
Recognizes a 60kD non-glycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker of the mitochondria in subcellular fractions. Species Crossreactivity: Crossreacts with Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.