		Technical Data
M3990-04	Mitochondrial Antiviral Signaling Protein (MAVS, VISA)

Description:
The mitochondrial antiviral signaling protein (MAVS, VISA) contributes to innate immunity by triggering IRF-3 and NF- B activation in response to viral infection, leading to the production of IFN- (1). The MAVS protein contains an N-terminal CARD domain and a C-terminal mitochondrial transmembrane domain. The MAVS adaptor protein plays a critical and specific role in viral defenses (2). MAVS acts downstream of the RIG-I RNA helicase and viral RNA sensor, leading to the recruitment of IKK , TRIF and TRAF6 (3,4). Some viruses have evolved strategies to circumvent these innate defenses by using proteases that cleave MAVS to prevent its mitochondrial localization (5,6).

Recommended Dilution:
Western Blot: 1:1000
Immunofluorescence (IF-IC): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide (KLH-coupled) corresponding to residues at the carboxyl terminus of human MAVS

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA and 50% glycerol.

Specificity:

Detects endogenous levels of total human MAVS protein. Bands detected at 52 and 75kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Seth, R.B. et al. (2005) Cell 122, 669-682.2. Sun, Q. et al. (2006) Immunity 24, 633-642.3. Xu, L.G. et al. (2005) Mol. Cell 19, 727-740.4. Yoneyama, M. et al. (2004) Nat. Immunol. 5, 730-737. 5. Lin, R. et al. (2006) J. Virol. 80, 6072-6083. 6. Chen, Z. et al. (2007) J. Virol. 81, 964-976.