Technical Data
M4470-01
Monocarboxylate Transporter 1 (MCT1, MOT1)
Description:
Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner. MCT1 (also known as MOT1 or SLC16A1 or MEV; mouse 493aa, rat 494aa, human 500aa; ~95% identity) is a membrane protein containing 12 transmembrane proteins. MCT1 is most closely related to MCT2 (~65% identity, whereas homolog with other MCT2-MCT8 isoforms is less (~35-53%). MCT1 has very wide tissue distribution. MCT1/MOT1 is ubiquitously expressed but is especially prominent in heart and red muscle. It is upregulated in response to increased work, suggesting an important role in lactic acid oxidation. It is the major isoform in tumor cell and erythrocytes.
Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-5ug/ml using ECL.
Immunohistochemistry: 3-10ug/ml. neutral buffered formaldehyde fixed tissue.
ELISA: 1:10,000-1:100,000 using 50-100ng control peptide (Cat #M4470-03E )/well
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
A synthetic peptide corresponding to 19aa near the C-terminus, cytoplasmic domain of human MCT1, conjugated to KLH. Species Sequence Homology: hamster; 63%, mouse; 57%
Purity:
Purified by immunoaffinity chromatography
Form
Supplied as a lyophilized powder from PBS, pH 7.5, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes human Monocarboxylate Transporter 1 (MCT1).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Wang, Q. et al., (2007) Pharm Res. 24:1067-1078. 1. Brauchi S 2004 Am J Physiol Cell Physiol, 288, 523-534 KINETICS, MOLECULAR BASIS AND DIFFERENTIATION OF L(+)-LACTATE TRANSPORT IN SPERMATOGENIC CELLS-IHC. 2. Buyse M 2002 J Biol. Chem 277, 28182 Luminal leptin enhances CD147/MCT-1 mediated uptake of butyrate in the human intestinal cell line Caco2-BBE-IF. 3. Am. J. Physiology Endocriol. Metabol. 36: E207-E213 (1997). 4. Diabetologia 42: 870-877 (1999). 5. J. Biol. Chem. 274: 284220 (1999). 6. Takanaga, H., et al., BBRC 217: 370-377 (1995). 7. Carpenter, L., et al., Biochim. Biophys. Acta 1279: 157-163 (1996). 8. Koehler-Stec, E.M., et al., Am. J. Physiol. 275: E516-E524 (1998). 9. Yoon, H., et al., Genomics 60: 366370 (1999).