Technical Data
M4470-02
Monocarboxylate Transporter 1 (MCT1, MOT1)
Description:
Monocarboxylates such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid, followed by an increase in pH, leading to inhibition of glycolysis. Some tissues, such as brain, heart and red skeletal muscle, readily oxidize lactic acid. These tissues must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner.

Applications:
Suitable for use in ELISA, Western Blot., Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-5ug/ml using Chemiluminescence.
ELISA: 1:10,000-1:100,000. Control peptide (Cat #M4470-02A) can be used to coat ELISA plates at 50-100ng per well.
Immunohistochemistry: 3-10ug/ml in neutral buffered formaldehyde fixed tissue
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 17aa sequence within the cytoplasmic C-terminus of rat MCT1, conjugated to KLH. Species Sequence Homology: hamster: 93%; mouse: 80%, human MCT1; 40%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity:
Recognizes rat Monocarboxylate Transporter 1 (MCT1). No significant sequence homology with other MCTs.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application references: 1. Wang, Q. and Morris, M.E. (2007) Mol. Pharm. 35:1393-1399. 2. Wang, Q. et al., (2006) Mol. Pharm. 3:675-685. 1. Am. J. Physiology Endocriol. Metabol. 36: E207-E213 (1997). 2. Diabetologia 42: 870-877 (1999). 3. J. Biol. Chem. 274: 284220-284220 (1999). 4. Takanaga, H., et al, BBRC 217: 370-377 (1995). 5. Carpenter, L., et al., Biochim. Biophys. Acta 1279: 157-163 (1996). 6. Koehler-Stec, E.M., et al., Am. J. Physiol. 275: E516-E524 (1998). 7. Yoon, H., et al., Genomics 60: 366-370 (1999). 8. Halestrap, A.P., Price, N.T., Biochem J. 343: 281-299 (review) (1999).