Technical Data
M4470-02B
Monocarboxylate Transporter 1 (MCT1)
Description:
Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid, followed by an increase in pH, leading to inhibition of glycolysis. Some tissues, such as brain, heart and red skeletal muscle, readily oxidize lactic acid. These tissues must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner. MCT1 (also known as MOT1 or SLC16A1 or MEV; mouse 493aa, rat 494aa, human 500aa; ~ 95 % identity) is a membrane protein containing 12 transmembrane proteins. MCT1 is most closely related to MCT2 (~65% identity, whereas homolog with other MCT2-MCT8 isoforms is less (~35-53%). MCT1 has very wide tissue distribution. MCT1/MOT1 is ubiquitously expressed. It is especially prominent in heart and red muscle. It is upregulated in response to increased work, suggesting an important role in lactic acid oxidation. It is the major isoform in tumor cell and erythrocytes.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000-1:100,000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceRatRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to 17aa within the C-terminal, cytoplasmic domain of rat MCT1, cnjugated to KLH. Species Sequence Homology: hamster; 93%, mouse; 80%, human MCT1; 40%.
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes rat Monocarboxylate Transporter 1 (MCT1). No significant sequence homology with other MCTs.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application references: 1. Wang, Q. and Morris, M.E. (2007) Mol. Pharm. 35:1393-1399. 2. Wang, Q. et al., (2006) Mol. Pharm. 3:675-685.1. Am. J. Physiology Endocriol. Metabol. 36: E207-E213 (1997). 2. Diabetologia 42: 870-877 (1999). 3. J. Biol. Chem. 274: 284220-284220 (1999). 4. Takanaga, H., et al, BBRC 217: 370-377 (1995). 5. Carpenter, L., et al., Biochim. Biophys. Acta 1279: 157-163 (1996). 6. Koehler-Stec, E.M., et al., Am. J. Physiol. 275: E516-E524 (1998). 7. Yoon, H., et al., Genomics 60: 366-370 (1999). 8. Halestrap, A.P., Price, N.T., Biochem J. 343: 281-299 (review) (1999).