Technical Data
M4470-03C
Monocarboxylate Transporter 1 (MCT1)
Description:
Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner.

MCT1 (also known as MOT1 or SLC16A1 or MEV; mouse 493aa, rat 49 aa, human 500aa; ~95% identity) is a membrane protein containing 12 transmembrane proteins. MCT1 is most closely related to MCT2 (~65% identity, whereas homolog with other MCT2-MCT8 isoforms is less (~35-53%). MCT1 has very wide tissue distribution. MCT1/MOT1 is ubiquitously expressed but is especially prominent in heart and red muscle. It is upregulated in response to increased work, suggesting an important role in lactic acid oxidation. It is the major isoform in tumor cell and erythrocytes.

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-5ug/ml (ECL).
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
A 19-aa peptide sequence within the cytoplasmic, C-terminus of human MCT1 (1) was synthesized, coupled to KLH
Purity:
Serum
Form
Supplied as a liquid, neat serum, 40% glycerol.
Specificity:
Human sequence is 63% conserved in hamster and 57% in mouse. No significant sequence homology was found with other MCTs. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking (use 5-10 ug per 1 ul of antiserum or 1 ug of aff pure IgG) to confirm antibody specificity
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Am. J. Physiology Endocriol. Metabol. 1997) 36, E207-E213; Diabetologia (1999) 42, 870-877; J. Biol. Chem. (1999) 274, 284220-284220; Takanaga H et al (1995) BBRC 217, 370-377; Carpenter L et al (1996) Biochim. Biophys. Acta 1279, 157-163; Koehler-Stec EM et al (1998) AM. J. Physiol. 275, E516-E524; Yoon H et al (1999) Genomics 60, 366-370; Halestrap AP and Price NT (1999) Biochem J. 343, 281-299 (review).