Technical Data
M4470-11
Monocarboxylate Transporter 2 (MCT2, MOT2)
Description:
Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner.

Applications:
Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Western Blot (Chemiluminescence): 1-5ug/ml.
Immunohistochemistry: 5-10ug Paraformaldehyde fixed.
Optimal dilutions to be determined by the researcher.

Control Peptide:
M4470-11A: Monocarboxylate Transporter 2, Rat Control Peptide

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
A 15aa peptide sequence near the C-terminus of rat MCT2 conjugated to KLH (Q63344). Species sequence homology: mouse (93%), hamster (66%).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophillized powder from PBS, pH 7.4, 0.1% BSA. Reconstitute in 100ul of PBS.
Specificity:
Recognizes rat Monocarboxylate Transporter 2 (MCT2). The immunogen shows no significant sequence homology with other MCT.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application references: 1. Wang, Q. and Morris, M.E. (2007) Mol. Pharm. 35:1393-1399. 2. Wang, Q. et al., (2006) Mol. Pharm. 3:675-685. 1. Brauchi S 2004 Am J Physiol Cell Physiol, 288, 523-534 IHC 2. Graham C 2007 Am J Physiol Regul Integr Comp Physiol. 2007 Feb;292(2):R997-1007. Epub 2006 Sep 28 IF 3. Jackson VN et al (1997) Biochem. J. 324:447453; 4. Dao L et al (1998) J. Biol. Chem. 273:2895928965; 5. Koehler-Stec EM et al (1998) Am. J. Physiol. 275:E516-E524; 6. Price NT et al (1998) Biochem. J. 329:321328 (review); 7. Halestrap AP and Price NT (1999) Biochem J. 343:281299 (review).