Technical Data
M4470-11B
Monocarboxylate Transporter 2 (MCT2)
Description:
Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner.

MCT2/MOT2 (mouse 484 aa, rat 489 aa, human 478 aa, chromosome 12q13) is less widely distributed than MCT. It is associated with tissues that demonstrate a high uptake affinity for lactate and pyruvate such as the kidney and liver (for gluconeogenesis) and neurons (for oxidation). It may function in transporting lactate from intestine and erythrocytes. MCT1 is most closely related to MCT1 (~55% identity, whereas homolog with other MCT1-MCT8 isoforms is less (~35-53%). Both N and C-termini are predicted to be cytoplasmic.

Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blotting 1-5ug/ml for affinity pure using Chemiluminescence technique.

ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
As reported
Immunogen:
A 15-aa peptide sequence within the cytoplasmic, C-terminus of rat MCT2 (1) was synthesized, coupled to KLH
Purity:
Serum
Form
Supplied as a liquid, neat serum, 40% glycerol.
Specificity:
Rat sequence is 93% conserved in mouse MCT2. No significant sequence homology exists with either human MCT2 or other MCTs. For human MCT2, we recommend the use of antibody feline # MCT22-S. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking (use 5-10 ug per 1 ul of antiserum or 1 ug of aff pure IgG) to confirm antibody specificity
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application references: 1. Wang, Q. and Morris, M.E. (2007) Mol. Pharm. 35:1393-1399. 2. Wang, Q. et al., (2006) Mol. Pharm. 3:675-685.(1) Jackson VN et al (1997) Biochem. J. 324, 447-453; Dao L et al (1998) J. Biol. Chem. 273, 28959-28965; Koehler-Stec EM et al (1998) Am. J. Physiol. 275, E516-E524; Price NT et al (1998) Biochem. J. 329, 321-328 (review); Halestrap AP and Price NT (1999) Biochem J. 343, 281-299 (review).