Technical Data
Monocarboxylate Transporter 3 (MCT3)
Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner. MCT3/REMP/SLC16A8 (mouse/rat 492-aa, human 504-aa; chromosome 22q12.3-q13.2) is exclusively located in the basal membrane of RPE, in contrast with MCT1, which was found on the apical surface. According to new nomenclature, the old MCT3 has now been reclassified as MCT4. Please consult the main MCT page for details on the new nomenclature.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
50ul4°C (-20°C Glycerol)Blue IceRatRabbit
Not determined
Senthetic peptide of 17-aa sequence within the cytoplasmic, C-terminus of rat MCT3, coupled to KLH
Supplied as a liquid in 0.05% sodium azide before the addition of 40% glycerol.
Recognizes rat MCT3. Sequence is 100% conserved in mouse MCT3. No significant sequence homology exists with either human MCT3 or other MCTs. Related Products: For human MCT3, we recommend the use of antibody feline #M4470-21B. Control peptide, because of its low MW (< 3kD), is not suitable for Western. It should be used for ELISA or antibody blocking (use 5-10ug per 1ul of antiserum) to confirm antibody specificity.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jackson, V.N., et al., (1997) Biochem. J. 324: 447-453. 2. Dao, L., et al., (1998) J. Biol. Chem. 273: 28,959-28,965. 3. Koehler-Stec, E.M., et al., (1998) Am. J. Physiol. 275: E516-E524. 4. Price, N.T., et al., (1998) Biochem. J. 329: 321-328 (review). 5. Halestrap, A.P. and Price, N.T. (1999) Biochem J. 343: 281-299 (review).