Technical Data
M4470-21B
Monocarboxylate Transporter 3 (MCT3)
Description:
Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner. MCT3/REMP/SLC16A8 (mouse/rat 492-aa, human 504-aa; chromosome 22q12.3-q13.2) is exclusively located in the basal membrane of RPE, in contrast with MCT1, which was found on the apical surface. According to new nomenclature, the old MCT3 has now been reclassified as MCT4. Please consult the main MCT page and Halestrap AP and Price NT (1999) Biochem J. 343, 281-299 (review) for a detailed nomenclature.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000 using 50-100ng of M4470-21A control peptide/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to a 14aa sequence within the cytoplasmic, C-terminus of human MCT3 (KLH).
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes human Monocarboxylate Transporter 3 (MCT3). Species sequence homology: Not suitable for use in rat and mouse. For rat and mouse crossreactivity use cat# M4470-20A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Philip NJ et al (1998) Am. J. Physiol. 274, R1824-R1828; 2. Philip NJ et al (1998) Am. J. Physiol. 280, C1319-C1326; 3. Yoon H et al (19999) Genomics 60, 366-370; 4. Price NT et al (1998) Biochem. J. 329, 321-328 (review); 5. Halestrap AP and Price NT (1999) Biochem J. 343, 281-299 (review).