Technical Data
Monocarboxylate Transporter 4 (MCT4, MOT4)
Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end-product of glycolysis. Some tissues, such as white skeletal muscle and red blood cells use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain/maintain a high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues such as brain, heart and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked monocarboxylate transporters (MCTs) located at the plasma membrane. At least 9 MCTs (MCT1-9)-related proteins have been identified in mammals that are expressed in a tissue specific manner.
MCT4/MOT4 is most closely related to MCT3. It is prominently expressed in skeletal muscle and other cells with a high glycolytic rate such as tumor cells and white blood cells, suggesting an important role in lactic acid efflux.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000-1:100,000 using 50-100ng of M4470-30F control peptide/well.
Western Blot: 1-5ug/ml using ECL.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
A synthetic peptide corresponding to 19aa near the C-terminal, cytoplasmic domain 4 of human Monocarboxylate Transporter 4, conjugated to KLH. Species Sequence Homology: mouse, 83%
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Recognizes human Monocarboxylate Transporter 4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
USBio Application References: 1. Wang, Q. and Morris, M.E., (2007) Drug Metabolism and Distribution 35: 1393-1399. 2. Wang, Q., et al., Pharm. Res. 24: 1067-1078 (2007). General References: 1. Wilson, M.C., et al., JBC 273: 15,920-15,926 (1998). 2. Price, N.T., et al., Biochem. J. 329: 321-328 (1998) (review). 3. Halestrap, A.P. and Price, N.T., Biochem J. 343: 281-299 (1999) (review).