Technical Data
M4470-50
Monocarboxylate Transporter 6 (MCT6, MOT6)
Description:
Monocarboxylates such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues. such as white skeletal muscle and red blood cells use this pathway to generate most of their ATP under normal pysiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain and maintain a high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular alctic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton linked membrane transporters called monocarboxylic acid transporters (MCTs). MCT6 is highly expressed in human kidney and placenta.

Applications:
Suitable for use in Western Blot and ELISA. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1-5ug/ml using ECL.
ELISA: 1:10,000-1:100,000 use 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.

Control Peptide: M4470-50B; Monocarboxylate Transporter 6, Human (MCT6)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide consisting of 20 amino acids within the cytoplasmic, C-terminus of human MCT6 (KLH).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Specificity:
Recognizes human Monocarboxylate Transporter 6 (MCT6/MOT6). Species sequence homology: mouse, 70%; rat, 65%. No significant sequence homology with other MCT.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Price NT et al (1998) Biochem. J. 329:321328 (review); Halestrap AP and Price NT (1999) Biochem J. 343:281299 (review).