Technical Data
Monocarboxylate Transporter 8 (MCT8, XPCT)
Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner. MCT8 or XPCT (X-linked PEST Containing Transporter; (mouse, 545 aa, human 613/598 aa, chromosome xq13.2) is highly expressed in liver, heart, and kidney.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.

Control Peptide:
M4470-70B: Monocarboxylate Transporter 8, Human Control Peptide (MCT8, XPCT)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
50ul-20CBlue IceMouseRabbit
Not determined
A 19aa synthetic peptide corresponding to within the Cytoplasmic, C-terminus of mouse MCT8 coupled to KLH. Species sequence homology: rat-94%, human-89%.
Supplied as a liquid in PBS, 0.05% sodium azide.
Recognizes mouse Monocarboxylate Transporter 8.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Price NT et al (1998) Biochem. J. 329, 321-328 (review); Halestrap AP and Price NT (1999) Biochem J. 343, 281-299 (review).